Prof. Dr. Utz Fischer

Prof. Dr. Utz Fischer

 
Phone:
     +49 931 - 31 84029
 
 
E-mail:

     utz.fischer@biozentrum.uni-wuerzburg.de


Room:
    
B128

 

 

  


   

Curriculum vitae

 

Name

Prof. Dr. Utz Fischer

 

Position

Professor and Chairman

Affiliation

 

Chair of Biochemistry, Theodor-Boveri-Institute at the Biocentre
University of Würzburg
Am Hubland
97074 Würzburg
Phone +49 931 - 31 84029

 

utz.fischer@biozentrum.uni-wuerzburg.de

Born

12.10.1964

 

 

 

 

 

 

Career

1983-1988

Studies in Biochemistry at the Free University of Berlin, Germany, Diploma degree

 

1989-1992

Dissertation at the Philipps-University Marburg in the group of Prof. R. Lührmann

 

1992-1995

Post-Doc, Institute of Molecular Biology and Tumor Research, University of Marburg, Germany

 

1995-1997

AIDS Scholar at the HHMI, University of Pennsylvania, Philadelphia (PA, USA)

 

1997-2003

Group leader at the MPI of Biochemistry, Martinsried, Germany

 

since 2003

Professor and Chair of Biochemistry, University of Würzburg, Germany

 

2008-2009

Speaker of the Biocentre/Theodor-Boveri-Institute, University of Würzburg, Germany

 

 

 

 

 

 

Research Fields

 

Structure and Function of macromolecular machines

RNA-metabolism and neuronal diseases

 

 

 

 

Professional Activities

Since 2007: Speaker of the DFG-Forschergruppe FOR855 “Cytoplasmic regulation of gene expression”

 

 

 

 

 

 

Awards

1989: Dissertation award of Hoechst Industries
1993: AIDS Scholar of the DKFZ (Heidelberg)
1993: Falcon Research Award of the German Society of Cell Biology
1998: Rhone-Poulenc Rorer Research Award
1999: Gerhard Hess Award of the DFG

  

  

  

  

  


 

Publications

  

Original Manuscripts

  

Linder B, Dill H, Hirmer A, Brocher J, Lee GP, Mathavan S, Bolz HJ, Winkler C, Laggerbauer B and Fischer U (2011): Systemic splicing factor deficiency causes tissue-specific defects: a zebrafish model for retinitis pigmentosa. Hum Mol Genet 20(2): 368-77.

 

Guderian G, Peter C, Wiesner J, Sickmann A, Schulze-Osthoff K, Fischer U and Grimmler M (2010): RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln or binding and modulates PRMT5 complex composition and substrate specificity. J Biol Chem 286(3): 1976-86.

 

Grimm C, Chari A, Reuter K and Fischer U (2010): A crystallization screen based on alternative polymeric precipitants. Acta Crystallogr D Biol Crystallogr. 66: 685-697.

 

Schäffler K, Schulz K, Hirmer A, Wiesner J, Grimm M, Sickmann A and Fischer U (2010): A stimulatory role for the La-related protein 4B in translation. RNA 16: 1488-99.

 

Martin G, Ostareck-Lederer A, Chari A, Neuenkirchen N, Dettwiler S, Blank D, Rüegsegger U, Fischer U and Keller W (2010): Arginine methylation in subunits of mammalian pre-mRNA cleavage factor I. RNA 16: 1646-59.

  

Galao RP*, Chari A*, Alves-Rodriguez I, Lobao D, Mas A, Kambach C, Fischer U and Diez J (2010): LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication. RNA 16: 817-827.
*these authors contributed equally to this work

 

Kroiss M, Brünger KM, Wiesner J, Grimmler M, Sickmann A and Fischer U (2009): Native purification of protein and RNA-protein complexes using a novel affinity procedure. Fly 3(3): 221-8.

  

Scheller N, Mina LB, Galão RP, Chari A, Giménez-Barcons M, Noueiry A, Fischer U, Meyerhans A and Diez J (2009): Translation and replication of hepatitis C virus genomic RNA depends on ancient cellular proteins that control mRNA fates. Proc Natl Acad Sci USA 106(32): 13517-22.

 

Guenther UP, Handoko L, Laggerbauer B, Jablonka S, Chari A, Alzheimer M, Ohmer J, Plöttner O, Gehring N, Sickmann A, von Au K, Schuelke M and Fischer U (2009): IGHMBP2 is a ribosome-associated helicase inactive in the neuromuscular disorder distal SMA type 1 (DSMA1). Hum Mol Genet 18(7): 1288-300.

  

Chari A, Golas M, Neuenkirchen N, Klingenhäger M, Sander B, Englbrecht C, Sickmann A, Stark H and Fischer U (2008): An assembly chaperone collaborates with the SMN complex to generate spliceosomal SnRNPs. Cell 135: 497-509.

  

Linder B, Plöttner O, Kroiss M, Hartmann E, Laggerbauer B, Meister G and Fischer U (2008): TDRD3 is a novel stress granule-associated protein ineracting with the Fragile-X syndrome protein FMRP. Hum Mol Genet 17: 3236-46.

  

Kroiss M, Schultz J, Wiesner J, Chari A, Sickmann A and Fischer U (2008): Evolution of an RNP assembly system: a minimal SMN-complex facilitates formation of U snRNPs in Drosophila melanogaster. Proc Nat Acad Sci USA 105: 10045-10050.

    

Markert A, Grimm M, Martinez, J, Wiesner J, Meyerhans A, Meyuhas O, Sickmann A and Fischer U (2008): The La-related protein, Larp7, is a component of the 7SK snRNP and affects Tat-dependent transcription of HIV-1. EMBOrep 9: 569-575.

  

Petri S, Grimmler M, Over S, Fischer U and Gruss O (2007): Dephosphorylation of survival motor neurons (SMN) by PPM1G/PP2Cgamma governs Cajal body localization and stability of the SMN-complex. J Cell Biol 179: 451-465.

  

Menzel N, Chari A, Fischer U, Linder M and Raabe T (2007): A 5’-fluorosulfonylbenzoyladenosine-based method to identify physiological substrates of a Drosophila p21-activated kinase.  Anal Biochem 368: 178-184.

 

Otter S, Grimmler M, Neuenkirchen N, Chari A, Sickmann A and Fischer U (2007): A comprehensive interaction map of the human survival of motor neuron (SMN) complex. J Biol Chem 282: 5825-5833.

  

Grimmler M, Otter S, Peter C, Müller F, Chari A and Fischer U (2005): Unrip, a factor implicated in cap-independent translation, associates with the cytosolic SMN complex and influences its intracellular localization. Hum Mol Genet 14: 3099-3111.

 

Azzouz TN, Pillai RS, Dapp C, Chari A, Meister G, Kambach C, Fischer U and Schumperli D (2005): Towards an assembly line for U7 snRNPs: interactions of U7-specific Lsm proteins with PRMT5 and SMN complexes. J Biol Chem 280: 34435-34440.

 

Winkler C, Eggert C, Gradl D, Meister G, Giegerich M, Wedlich D and Fischer U (2005): Reduced RNP assembly causes motor axon degeneration in an animal model for spinal muscular atrophy. Genes&Dev 19: 2320-2330.

  

Grimmler M, Martin L, Nousiainen M, Koerner R, Meister G and Fischer U (2005): Phosphorylation regulates the activity of the SMN-complex during assembly of spliceosomal U snRNPs. EMBOrep 6: 70-76.

 

Grohmann K, Rossoll W, Kobsar I, Holtmann B, Jablonka S, Stoltenberg-Didinger G, Hübner C, Fischer U, Martini R and Sendtner M (2004): Characterization of Ighmbp2 in motor neurons and implications for the pathomechanism in a mouse model for human spinal muscular atrophy with respiratory distress type 1 (SMARD1). Hum Mol Genet 15: 2031-2042.

  

Sun Y, Grimmler M, Schwarzer V, Schoenen F, Fischer U and Wirth B (2004): Molecular and functional analysis of intragenic SMN1 mutations in patients with spinal muscular atrophy. Hum Mutations 25: 64-71.

 

Malatesta M, Scassellati C, Meister G, Plöttner O, Bühler D, Sowa G, Martin TE, Keidel E, Fischer U and S Fakan (2004): Ultrastructural characterisation of a nuclear domain highly enriched in survival of motor neuron (SMN) protein. Exp Cell Res 292 (2): 312-321.

 

Pillai R, Grimmler M, Meister G, Will CL, Lührmann R, Fischer U and Schümperli D (2003): Unique Sm core structure of U7 snRNPs: assembly by a specialized SMN-complex and role of a new component, Lsm11, in histone RNA processing. Genes&Dev 17: 2321-2333.

 

Barth M, Meister G, Fischer U and Grässer F (2003): Epstein-Barr virus nuclear antigen 2 binds via its methylated arginine-glycine repeat to the survival motor neuron protein. J Virol 77: 5008-5014.

 

Meister G and Fischer U (2002): Assisted RNP assembly: SMN- and PRMT5-complexes cooperate in the formation of spliceosomal U snRNPs. EMBO J 21: 5853-5863.

  

Jablonka S, Holtmann B, Meister G, Bandilla M, Russoll W, Fischer U and Sendtner M (2002): Gene targeting of Gemin2 in mice reveals a correlation between defects in the biogenesis of U snRNPs and motor neuron cell death. Proc Natl Acad Sci USA 99: 10126-10131.

  

Meister G, Eggert C, Bühler D, Brahms H, Kambach C and Fischer U (2001): Methylation of Sm proteins and regulation of U snRNP assembly by a complex containing PRMT5 and pICln. Current Biol 11: 1990-1994.

 

Brahms H, Meheus L, de Brabandere V, Fischer U and Lührmann R (2001): Symmetrical dimethylation of arginine residues in spliceosomal Sm protein B/B‘ and the Sm-like protein LSm4, and their interaction with the SMN protein. RNA 7(11): 1531-42.

 

Jones KW, Gorzynski K, Hales CM, Fischer U, Badbanchi F, Terns RM and Terns MP (2001): Direct interaction of the spinal muscular atrophy disease protein SMN with the core snoRNP protein fibrillarin. J Biol Chem 276(42): 38645-51.

 

Meister G, Bühler D, Pillai R, Lottspeich F and Fischer U (2001): A multiprotein complex mediates the ATP-dependent assembly of spliceosomal U snRNPs. Nat Cell Biol 3: 945-949.

 

Jablonka S, Bandilla M, Wiese S, Bühler D, Wirth B, Sendtner M and Fischer U (2001): Co-regulation of survival of motor neuron (SMN) protein and its interactor SIP1 during development and in spinal muscular atrophy. Hum Molec Genetics 10: 497-505.

 

Laggerbauer B, Ostareck D, Keidel E-M, Ostareck-Lederer A and Fischer U (2001): Evidence that fragile X mental retardation protein is a negative regulator of translation. Hum Molec Genetics 10: 329-338.

  

Meister G, Hannus S, Plöttner O, Laggerbauer B, Baars T, Hartmann E, Fakan S and Fischer U (2001): SMNrp is an essential pre-mRNA splicing factor required for the formation of the mature spliceosome. EMBO J 20: 2304-2314.

 

Meister G, Bühler D, Laggerbauer B, Zobawa M, Lottspeich F and Fischer U (2000): Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins. Hum Molec Genetics 9: 1977-1986.

  

Selenko P, Sprangers O, Stier R, Bühler,G, Fischer U and Sattler M (2001): Structure and Sm protein interaction of the SMN Tudor domain. Nat Struct Biol 8: 27-31.

 

Hannus S, Seraphin B and Fischer U (2000): The Schizosaccharomyces pombe protein Yab8p and a novel factor, Yip1p, share structural and functional similarity with the Spinal Muscular Atrophy-associated proteins SMN and SIP1. Hum Molec Genetics 9: 663-674.

 

Bühler D, Raker V, Lührmann R and Fischer U (1999): Essential role for the Tudor domain of SMN in U snRNP assembly: implications for Spinal Muscular Atrophy. Hum Molec Genetics 8: 2351-2357.

  

Fischer U, Lührmann R, Dreyfuss G and Malim MH (1999): HIV-1 Rev accesses a non-mRNA export pathway that overrides nuclear retention of RNA. Nucleic Acids Res 27: 4128-4134.

  

Filali M, Qiu J, Awasthi S, Fischer U, Monos D and Kamoun M (1999): Monoclonal antibody specific to a subclass of polyproline-Arg motif provides evidence for the presence of an snRNA-free spliceosomal Sm protein complex in vivo: implications for molecular interactions involving proline-rich sequences of Sm B/B' proteins. J Cell Biochem 74: 168-180.

 

Malatesta M, Fakan S and Fischer U (1999): The Sm core domain mediates targeting of U1 snRNP in subnuclear compartments involved in transcription and splicing. Exp Cell Res 249: 189-198.

 

Fouchier RAM, Meyer BE, Simon, JHM, Fischer U, Albright AV, Gonzales-Scavano F and Malim MH (1998): Interaction of HIV-1 Vpr with the nuclear pore complex. J Virol 72: 6004-6013.

 

Fouchier RAM, Meyer BE, Simon JHM, Fischer U and Malim MH (1997): HIV-1 infection in non-dividing cells: evidence that the viral matrix protein is important for Gag processing but not for post-entry nuclear import. EMBO J 16: 4531-4539.

 

Lui Q, Fischer U and Dreyfuss G (1997): The spinal muscular atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins. Cell 90: 1013-1021.

 

Fischer U, Lui Q and Dreyfuss G (1997): The SMN-SIP1 complex has an essential role in spliceosomal U snRNP biogenesis. Cell 90: 1023-1029.

 

Izaurralde E, Jarmolowski A, Beisel C, Mattaj IW, Dreyfuss G and Fischer U (1996): A role for the hnRNP A1 M9-transport signal in mRNA nuclear export. J Cell Biol 137: 27-35.

  

Sekine M, Kadokura M, Satoh T, Seio K, Wada T, Fischer U, Sumpter V and Lührmann R (1996): Chemical synthesis of a 5’-terminal TMG-capped ribonucleotide m32,2,7G5'pppAmpUmpA of U1 RNA. J Organic Chemistry 61: 4412-4422.

 

Fridell R, Fischer U, Lührmann R, Meyer BE, Meinkoth JL, Malim MH and Cullen BR (1996): Amphibian transcription factor IIIA proteins contain a sequence functionally equivalent to the nuclear export signal of human immunodeficiency virus type 1 Rev. Proc Natl Acad Sci USA 93: 2936-2940.

  

Fischer U, Huber J, Boelens W, Mattaj IW and Lührmann R (1995): The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell 82: 475-483.

  

Sekine M, Kadolura M, Satoh T, Seio K, Wada T, Fischer U and Lührmann R (1994): Chemical synthesis of the 5´-terminal structure of U1 RNA. Nucl Acid Symp Series 31: 61-62.

  

Kammerloher W, Fischer U, Piechottka GP and Schäffner AR (1994): Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system. The Plant J 6: 187-199.

 

Plessel G, Fischer U and Lührmann R (1994): m3G-cap hypermethylation of U1 small nuclear ribonucleoprotein (snRNP) in vitro: evidence that the U1 mall nuclear RNA-(Guanosine-N2)-methyltransferase is a non-snRNP cytoplasmic protein that requires a binding site on the Sm core domain. Mol Cell Biol 14: 4160-4172.

  

Fischer U, Meyer S, Teufel M, Heckel C, Lührmann R and Rautmann G (1994): Evidence that HIV-1 Rev directly promotes nuclear export of unspliced RNA. EMBO J 13: 4105-4112.

  

Hackl W, Fischer U and Lührmann R (1994): A 69-kD protein that associates reversibly with the Sm core domain of several spliceosomal snRNP species. J Cell Biol 124: 261-272.

  

Fischer U, Heinrich J, van Zee K, Fanning E and Lührmann R (1994): Nuclear transport of U1 snRNP in somatic cells: differences in signal requirement compared with Xenopus laevis oocytes. J Cell Biol 125: 971-980.

    

van Zee K, Dickmanns A, Fischer U, Lührmann R and Fanning E (1993): A cytoplasmically anchored nuclear protein interferes specifically with the import of nuclear proteins but not U1 snRNA. J Cell Biol 121: 229-240.

 

Fischer U, Sumpter V, Sekine M, Satoh T and Lührmann R (1992): Nucleo-cytoplasmic transport of U snRNPs: Definition of a nuclear location signal in the Sm core domain that binds a transport receptor independently of the m3G cap. EMBO J 12: 573-583.

 

Sumpter V, Kahrs A, Fischer U, Kornstädt U and Lührmann R (1992): In vitro reconstitution of U1 and U2 snRNPs from isolated proteins and snRNA. Mol Biol Report 16: 229-240.

  

Fischer U, Darzynkiewicz E, Tahara SM, Dathan NA, Lührmann R and Mattaj IW (1991): Diversity in the signals required for nuclear accumulation of U snRNPs and variety in the pathways of nuclear transport. J Cell Biol 113: 705-714.

  

Fischer U and Lührmann R (1990): An essential signalling role for the m3G cap in the transport of U1 snRNP to the nucleus. Science 249: 786-790.

  

Hornig H, Fischer U, Costas M, Rauh A and Lührmann R (1989): Analysis of genomic clones of the murine U1 RNA-associated 70-KDa proteins reveals a high evolutionary conservation of the protein between human and mouse. Eur J Biochem 182: 45-50.

  

 

 

 

 

 

  

 

 

Reviews and Book Chapter

 

Fischer U, Englbrecht C and Chari A (2011): Biogenesis of ribonucleoprotein particles involved in mRNA maturation. Wiley Interdisciplinary Reviews: RNA.

 

Chari A and Fischer U (2010): Cellular strategies for the assembly of molecular machines. Trends Biochem Sci 35: 676-683.

 

Chari A and Fischer U (2009): Molekulares Bodybuilding: Wie Zellen RNA-Proteinkomplexe herstellen. Biospektrum 15. Jahrgang, Ausgabe 05/09.

 

Chari A, Paknia E and Fischer U (2009): The role of RNP biogenesis in Spinal Muscular Atrophy. Curr Opin Cell Biol 21: 387-393.

 

Kroiss M, Fischer U and Schultz J (2009): When one plus one equals three: Biochemistry and bioinformatics combine to answer complex questions. Fly 3: 212-214.

 

Neuenkirchen N, Chari A and Fischer U (2008): Deciphering the assembly pathway of Sm-class U snRNPs. FEBS Lett 582: 1997-2003.

   

Fischer U, Kroiss M and Chari A (2007): Wenn Muskeln die Nerven verlieren. Biospektrum 13. Jahrgang, Ausgabe 06/07.

 

Eggert C, Chari A, Laggerbauer B and Fischer U (2006): Spinal muscular atrophy: the RNP connection. Trends Mol Med 12: 113-121.

 

Eggert C and Fischer U (2003): Der Spinalen Muskelatrophie auf der Spur. Bioforum 11/2003.

 

Eggert C and Fischer U (2003): RNA-Interferenz: ein neues Werkzeug zur Analyse der Genfunktion. Biospektrum 9. Jahrgang, Ausgabe 04/03.

 

Meister G, Eggert C and Fischer U (2002): SMN-mediated assembly of RNPs: a complex story. Trends in Cell Biol. 12: 1-7.

 

Fischer U, Hannus S and Plöttner O (1999): Searching for the cause of spinal muscular atrophy. Innovation 7: 4-6.

 

Nakielny S, Fischer U, Michael WM and Dreyfuss G (1997): RNA-transport. Annu Rev Neurosci 20: 269-301.

 

Liu Q, Siomi H, Siomi MC, Fischer U, Zhang Y, Wan L and Dreyfuss G (1996): Molecular characterization of the protein products of the Fragile X Syndrome gene and the Survival of Motor Neurons gene. Cold Spring Harbor Symp Quant Biol 61: 689-697.

   

Fischer U, Michael WM, Lührmann R and Dreyfuss G (1996): Signal-mediated nuclear export pathways of proteins and RNAs.Trends in Cell Biol 6: 290-293.