Chair of Biochemistry

    Publications

    K. Hipp, C. Grimm, H. Jeske and B. Bottcher (2017) Near-Atomic Resolution Structure of a Plant Geminivirus Determined by Electron Cryomicroscopy. Structure 25, 1303-1309 e3.

    R. Sundaramoorthy, A. L. Hughes, V. Singh, N. Wiechens, D. P. Ryan, H. El-Mkami, M. Petoukhov, D. I. Svergun, B. Treutlein, S. Quack, M. Fischer, J. Michaelis, B. Bottcher, D. G. Norman and T. Owen-Hughes (2017)  Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes. Elife  6,

    R. L. Cassady-Cain, E. A. Blackburn, H. Alsarraf, E. Dedic, A. G. Bease, B. Bottcher, R. Jorgensen, M. Wear and M. P. Stevens (2016)  Biophysical characterization and activity of lymphostatin, a multifunctional virulence factor of attaching & effacing Escherichia coli. J Biol Chem  291, 5803-5816.

    S. M. Currie, E. Gwyer Findlay, A. J. McFarlane, P. M. Fitch, B. Bottcher, N. Colegrave, A. Paras, A. Jozwik, C. Chiu, J. Schwarze and D. J. Davidson (2016)  Cathelicidins Have Direct Antiviral Activity against Respiratory Syncytial Virus In Vitro and Protective Function In Vivo in Mice and Humans. J Immunol  196, 2699-710.

    B. Böttcher, V. Prazak, A. Rasmussen, S. S. Black and T. Rasmussen (2015)  The Structure of YnaI Implies Structural and Mechanistic Conservation in the MscS Family of Mechanosensitive Channels. Structure  23, 1705-17014.

    H. Dong, P. Li, B. Böttcher, R. M. Elliott and C. Dong (2013)  Crystal structure of Schmallenberg orthobunyavirus nucleoprotein-RNA complex reveals a novel RNA sequestration mechanism. RNA  19, 1129-1136.

    B. Bradatsch, C. Leidig, S. Granneman, M. Gnadig, D. Tollervey, B. Bottcher, R. Beckmann and E. Hurt (2012)  Structure of the pre-60S ribosomal subunit with nuclear export factor Arx1 bound at the exit tunnel. Nat Struct Mol Biol  19, 1234-41.

    G. Guedez, K. Hipp, V. Windeisen, B. Derrer, M. Gengenbacher, B. Bottcher, I. Sinning, B. Kappes and I. Tews (2012)  Assembly of the Eukaryotic PLP-Synthase Complex from Plasmodium and Activation of the Pdx1 Enzyme. Structure  20, 172-84.

    K. Hipp, K. Galani, C. Batisse, S. Prinz and B. Bottcher (2012)  Modular architecture of eukaryotic RNase P and RNase MRP revealed by electron microscopy. Nucleic Acids Res  40, 3275-3288.

    S. Lebaron, C. Schneider, R. W. van Nues, A. Swiatkowska, D. Walsh, B. Bottcher, S. Granneman, N. J. Watkins and D. Tollervey (2012)  Proofreading of pre-40S ribosome maturation by a translation initiation factor and 60S subunits. Nat Struct Mol Biol  19, 744-53.

    D. C. Soares, N. J. Bradshaw, J. Zou, C. K. Kennaway, R. S. Hamilton, Z. A. Chen, M. A. Wear, E. A. Blackburn, J. Bramham, B. Bottcher, J. K. Millar, P. N. Barlow, M. D. Walkinshaw, J. Rappsilber and D. J. Porteous (2012)  The Mitosis and Neurodevelopment Proteins NDE1 and NDEL1 Form Dimers, Tetramers, and Polymers with a Folded Back Structure in Solution. J Biol Chem  287, 32381-93.

    C. Fernandez-Tornero, B. Böttcher, U. J. Rashid and C. W. Muller (2011)  Analyzing RNA polymerase III by electron cryomicroscopy. RNA Biol  8, 760-765.

    B. Gerlach, J. A. Kleinschmidt and B. Bottcher (2011)  Conformational Changes in Adeno-Associated Virus Type 1 Induced by Genome Packaging. J Mol Biol  409, 427-438.

    F. Nasertorabi, C. Batisse, M. Diepholz, D. Suck and B. Bottcher (2011)  Insights into the structure of the CCR4-NOT complex by electron microscopy. FEBS Lett  585, 2182-6.

    F. Sonntag, K. Kother, K. Schmidt, M. Weghofer, C. Raupp, K. Nieto, A. Kuck, B. Gerlach, B. Bottcher, O. J. Muller, K. Lux, M. Horer and J. A. Kleinschmidt (2011)  The assembly activating protein (AAP) promotes capsid assembly of different AAV serotypes. J Virol  85, 12686-12697.

    B. Bottcher and K. Hipp (2010)  Single-particle applications at intermediate resolution. Adv Protein Chem Struct Biol  81, 61-88.

    M. A. Brooks, K. Gewartowski, E. Mitsiki, J. Letoquart, R. A. Pache, Y. Billier, M. Bertero, M. Correa, M. Czarnocki-Cieciura, M. Dadlez, V. Henriot, N. Lazar, L. Delbos, D. Lebert, J. Piwowarski, P. Rochaix, B. Bottcher, L. Serrano, B. Seraphin, H. van Tilbeurgh, P. Aloy, A. Perrakis and A. Dziembowski (2010)  Systematic bioinformatics and experimental validation of yeast complexes reduces the rate of attrition during structural investigations. Structure  18, 1075-82.

    S. Ernst, C. Batisse, N. Zarrabi, B. Böttcher and B. Boersch (2010)  Regulatory assembly of the vacuolar proton pump VOV1-ATPase in yeast cells by FLIM-FRET. Proceedings of SPIE  7569,

    C. Fernandez-Tornero, B. Bottcher, U. J. Rashid, U. Steuerwald, B. Florchinger, D. P. Devos, D. Lindner and C. W. Muller (2010)  Conformational flexibility of RNA polymerase III during transcriptional elongation. EMBO J  29, 3762-72.

    D. Flemming, K. Thierbach, P. Stelter, B. Bottcher and E. Hurt (2010)  Precise mapping of subunits in multiprotein complexes by a versatile electron microscopy label. Nat Struct Mol Biol  17, 775-8.

    J. Batisse, C. Batisse, A. Budd, B. Bottcher and E. Hurt (2009)  Purification of nuclear poly(A)-binding protein Nab2 reveals association with the yeast transcriptome and a messenger ribonucleoprotein core structure. J Biol Chem  284, 34911-7.

    D. Flemming, P. Sarges, P. Stelter, A. Hellwig, B. Bottcher and E. Hurt (2009)  Two structurally distinct domains of the nucleoporin Nup170 cooperate to tether a subset of nucleoporins to nuclear pores. J Cell Biol  185, 387-95.

    T. Heitkamp, B. Bottcher and J. C. Greie (2009)  Solution structure of the KdpFABC P-type ATPase from Escherichia coli by electron microscopic single particle analysis. J Struct Biol  166, 295-302.

    S. Kuhner, V. van Noort, M. J. Betts, A. Leo-Macias, C. Batisse, M. Rode, T. Yamada, T. Maier, S. Bader, P. Beltran-Alvarez, D. Castano-Diez, W. H. Chen, D. Devos, M. Guell, T. Norambuena, I. Racke, V. Rybin, A. Schmidt, E. Yus, R. Aebersold, R. Herrmann, B. Bottcher, A. S. Frangakis, R. B. Russell, L. Serrano, P. Bork and A. C. Gavin (2009)  Proteome organization in a genome-reduced bacterium. Science  326, 1235-40.

    C. Ulbrich, M. Diepholz, J. Bassler, D. Kressler, B. Pertschy, K. Galani, B. Bottcher and E. Hurt (2009)  Mechanochemical removal of ribosome biogenesis factors from nascent 60S ribosomal subunits. Cell  138, 911-22.

    M. Cristodero, B. Bottcher, M. Diepholz, K. Scheffzek and C. Clayton (2008)  The Leishmania tarentolae exosome: purification and structural analysis by electron microscopy. Mol Biochem Parasitol  159, 24-9.

    M. Diepholz, M. Börsch and B. Böttcher (2008)  Structural organization of the V-ATPase and its implications for regulatory assembly and disassembly. Biochem Soc Trans  36, 1027-31.

    M. Diepholz, D. Venzke, S. Prinz, C. Batisse, B. Flörchinger, M. Rössle, D. I. Svergun, B. Böttcher and J. Fethiere (2008)  A different conformation for EGC stator subcomplex in solution and in the assembled yeast V-ATPase: possible implications for regulatory disassembly. Structure  16, 1789-98.

    T. Heitkamp, R. Kalinowski, B. Bottcher, M. Borsch, K. Altendorf and J. C. Greie (2008)  K+-translocating KdpFABC P-type ATPase from Escherichia coli acts as a functional and structural dimer. Biochemistry  47, 3564-75.

    T. Pohl, D. Schneider, R. Hielscher, S. Stolpe, K. Dorner, M. Kohlstadt, B. Bottcher, P. Hellwig and T. Friedrich (2008)  Nucleotide-induced conformational changes in the Escherichia coli NADH:ubiquinone oxidoreductase (complex I). Biochem Soc Trans  36, 971-5.

    C. Fernandez-Tornero, B. Bottcher, M. Riva, C. Carles, U. Steuerwald, R. W. Ruigrok, A. Sentenac, C. W. Muller and G. Schoehn (2007)  Insights into transcription initiation and termination from the electron microscopy structure of yeast RNA polymerase III. Mol Cell  25, 813-23.

    S. Seitz, S. Urban, C. Antoni and B. Bottcher (2007)  Cryo-electron microscopy of hepatitis B virions reveals variability in envelope capsid interactions. EMBO J  26, 4160-7.

    P. Stelter, R. Kunze, D. Flemming, D. Hopfner, M. Diepholz, P. Philippsen, B. Bottcher and E. Hurt (2007)  Molecular basis for the functional interaction of dynein light chain with the nuclear-pore complex. Nat Cell Biol  9, 788-96.

    B. Böttcher, M. Vogel, M. Ploss and M. Nassal (2006)  High plasticity of the hepatitis B virus capsid revealed by conformational stress. J Mol Biol  356, 812-22.

    T. Schafer, B. Maco, E. Petfalski, D. Tollervey, B. Bottcher, U. Aebi and E. Hurt (2006)  Hrr25-dependent phosphorylation state regulates organization of the pre-40S subunit. Nature  441, 651-5.

    C. Skamel, M. Ploss, B. Böttcher, T. Stehle, R. Wallich, M. M. Simon and M. Nassal (2006)  Hepatitis B virus capsid-like particles can display the complete, dimeric outer surface protein C and stimulate production of protective antibody responses against Borrelia burgdorferi infection. J Biol Chem  281, 17474-81.

    J. Fethiere, D. Venzke, D. R. Madden and B. Bottcher (2005)  Peripheral stator of the yeast V-ATPase: stoichiometry and specificity of interaction between the EG complex and subunits C and H. Biochemistry  44, 15906-14.

    S. Kronenberg, B. Bottcher, C. W. von der Lieth, S. Bleker and J. A. Kleinschmidt (2005)  A conformational change in the adeno-associated virus type 2 capsid leads to the exposure of hidden VP1 N termini. J Virol  79, 5296-303.

    M. Lutzmann, R. Kunze, K. Stangl, P. Stelter, K. F. Toth, B. Bottcher and E. Hurt (2005)  Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem  280, 18442-51.

    D. Venzke, I. Domgall, T. Köcher, J. Fethiere, S. Fischer and B. Böttcher (2005)  Elucidation of the stator organization in the V-ATPase of Neurospora crassa. J Mol Biol  349, 659-669.

    P. Aloy, B. Bottcher, H. Ceulemans, C. Leutwein, C. Mellwig, S. Fischer, A. C. Gavin, P. Bork, G. Superti-Furga, L. Serrano and R. B. Russell (2004)  Structure-based assembly of protein complexes in yeast. Science  303, 2026-9.

    B. Böttcher, S. Unseld, H. Ceulemans, R. B. Russell and H. Jeske (2004)  Geminate structures of African cassava mosaic virus. J Virol  78, 6758-65.

    J. Fethiere, D. Venzke, M. Diepholz, A. Seybert, A. Geerlof, M. Gentzel, M. Wilm and B. Bottcher (2004)  Building the stator of the yeast vacuolar-ATPase: specific interaction between subunits E and G. J Biol Chem  279, 40670-6.

    T. Friedrich and B. Bottcher (2004)  The gross structure of the respiratory complex I: a Lego System. Biochim Biophys Acta  1608, 1-9.

    C. Mellwig and B. Böttcher (2003)  A unique resting position of the ATP-synthase from chloroplasts. J Biol Chem  278, 18544-9.

    P. Aloy, F. D. Ciccarelli, C. Leutwein, A. C. Gavin, G. Superti-Furga, P. Bork, B. Bottcher and R. B. Russell (2002)  A complex prediction: three-dimensional model of the yeast exosome. EMBO Rep  3, 628-635.

    B. Böttcher, D. Scheide, M. Hesterberg, L. Nagel-Steger and T. Friedrich (2002)  A novel, enzymatically active conformation of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I). J Biol Chem  277, 17970-17977.

    I. Domgall, D. Venzke, U. Luttge, R. Ratajczak and B. Bottcher (2002)  Three-dimensional Map of a Plant V-ATPase Based on Electron Microscopy. J Biol Chem  277, 13115-13121.

    S. Kronenberg, J. A. Kleinschmidt and B. Bottcher (2001)  Electron cryo-microscopy and image reconstruction of adeno-associated virus type 2 empty capsids. EMBO Rep  2, 997-1002.

    C. Mellwig and B. Bottcher (2001)  Dealing with particles in different conformational states by electron microscopy and image processing. J Struct Biol  133, 214-20.

    G. Beterams, B. Bottcher and M. Nassal (2000)  Packaging of up to 240 subunits of a 17 kDa nuclease into the interior of recombinant hepatitis B virus capsids. FEBS Lett  481, 169-76.

    B. Böttcher (2000)  ATP synthase Is revolution effective? EMBO Reports  1, 223-224.

    B. Böttcher, I. Bertsche, R. Reuter and P. Gräber (2000)  Direct visualisation of conformational changes in EF0F1 by electron microscopy. J Mol Biol  296, 449-57.

    B. Böttcher and P. Gräber (2000)  The structure of the H+-ATP synthase from chloroplasts and its subcomplexes as revealed by electron microscopy. Biochim Biophys Acta  1458, 404-16.

    P. A. Kratz, B. Bottcher and M. Nassal (1999)  Native display of complete foreign protein domains on the surface of hepatitis B virus capsids. Proc Natl Acad Sci U S A  96, 1915-20.

    B. Böttcher, L. Schwarz and P. Gräber (1998)  Direct indication for the existence of a double stalk in CF0F1. J Mol Biol  281, 757-62.

    B. Böttcher, N. Tsuji, H. Takahashi, M. R. Dyson, S. Zhao, R. A. Crowther and K. Murray (1998)  Peptides that block hepatitis B virus assembly: analysis by cryomicroscopy, mutagenesis and transfection. Embo J  17, 6839-45.

    B. Böttcher, N. A. Kiselev, V. Y. Stel'Mashchuk, N. A. Perevozchikova, A. V. Borisov and R. A. Crowther (1997)  Three-dimensional structure of infectious bursal disease virus determined by electron cryomicroscopy. J Virol  71, 325-30.

    B. Bottcher, S. A. Wynne and R. A. Crowther (1997)  Determination of the fold of the core protein of hepatitis B virus by electron cryomicroscopy. Nature  386, 88-91.

    B. Böttcher, S. A. Wynne and R. A. Crowther (1997)  The fold of the core protein of hepatitis B virus determined by electron cryomicroscopy. Hitachi instrument news  32, 3-8.

    C. Menendez, A. Otto, G. Igloi, P. Nick, R. Brandsch, B. Schubach and B. Bottcher (1997)  Molybdate-uptake genes and molybdopterin-biosynthesis genes on a bacterial plasmid--characterization of MoeA as a filament-forming protein with adenosinetriphosphatase activity. Eur J Biochem  250, 524-31.

    B. Böttcher and R. A. Crowther (1996)  Difference imaging reveals ordered regions of RNA in turnip yellow mosaic virus. Structure  4, 387-94.

    Böttcher (1995)  Electron cryo-microscopy of graphite in amorphous ice. Ultramicroscopy  58, 417-424.

    B. Bottcher, P. Graber, E. J. Boekema and U. Lucken (1995)  Electron cryomicroscopy of two-dimensional crystals of the H+-ATPase from chloroplasts. FEBS Letters  373, 262-264.

    B. Böttcher, U. Lücken and P. Gräber (1995)  The structure of the H+-ATPase from chloroplasts by electron cryomicroscopy. Biochemical Society Transactions  23, 780-785.

    R. A. Crowther, N. A. Kiselev, B. Bottcher, J. A. Berriman, G. P. Borisova, V. Ose and P. Pumpens (1994)  Three-Dimensional Structure of Hepatitis B Virus Core Particles Determined by Electron Cryomicroscopy. Cell  77, 943-950.

    E. J. Boekema and B. Bottcher (1992)  The Structure of Atp Synthase from Chloroplasts - Conformational-Changes of Cf1 Studied by Electron-Microscopy. Biochimica Et Biophysica Acta  1098, 131-143.

    B. Böttcher, P. Gräber and E. J. Boekema (1992)  The structure of Photosystem I from the thermophilic cyanobacterium Synechococcus sp. determined by electron microscopy of two-dimensional crystals. Biochim Biophys Acta  1100, 125-36.

    Böttcher, Lücken, Boekema and Gräber (1990)  On the structure of the ATP-Synthase from Chloroplasts. Cur.Res.i.Photosynth  9, 33-36.

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